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high binding 96 well plates  (Greiner Bio)


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    Structured Review

    Greiner Bio high binding 96 well plates
    High Binding 96 Well Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/high+binding+96+well+plates/Microplate+96+Well+Ps+F-Bottom+Clear/pm41872358-228-0-3
    Average 97 stars, based on 860 article reviews
    high binding 96 well plates - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: An allergen immobilization platform based on material-binding peptides for highly sensitive detection of allergies.
    Article Snippet: .. Fig. 3B compares the performance of the described ELISA using Bet v 1-Snakin-1 on commercial high-binding 96-well plates (Greiner Bio-One 655061) with standard 96-well plates made from PS (Greiner Bio-One 655101). ..

    other:

    Article Title: Heterologous saRNA prime - multivalent protein boost strategy induces broad and durable immunity against SARS-CoV-2 and MERS-CoV.
    Article Snippet: SPR measurements were performed on a Biacore 3000 instrument (GE Healthcare) by NBS-C BioScience, Vienna Austria as previously described.77

    Recombinant:

    Article Title: Long-term immune responses to SARS-CoV-2 Omicron BA.4/5 mRNA booster in people living with HIV.
    Article Snippet: Total RBD-binding antibodies reactive against WT and BA.4/5 virus were measured in EDTA plasma samples by a homemade ELISA16,20,32. .. Briefly, high-binding 96-well plates (Greiner Bio-One, Cat# 655061) were coated with 3 μg/mL of recombinant wild-type SARS-CoV-2 receptor binding domain (RBD) [ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified), Cat# SPD-C52H3] or recombinant Omicron BA.4/5 SARS-CoV-2 RBD [ACROBiosystems SARS-CoV-2 Spike RBD Protein, His Tag (BA.4&BA.5/Omicron) (MALS verified), Cat# SPDC522r] diluted in 50mM of carbonate-bicarbonate buffer pH 9.6 (SigmaAldrich, Cat# C3041-50cap) and incubated overnight at+4 °C. .. Plates were then washed with washing buffer [PBS+ 0.05% TWEEN®20 (Sigma, Cat# P9416-100ML)] and blocked for 1.5 hours with blocking buffer [PBS+ 2% bovine serum albumin (BSA) (Sigma-Aldrich, Cat# A7030-100G)] at +37 °C.

    Article Title: Antigenic peptide delivery to antigen-presenting cells using a CD40-coiled coil affinity-based platform
    Article Snippet: Synthesized K coiled-coil peptides fused with a GS peptide linker and OVA peptides (KIAALKE) 3-6 -GS-SIINFEKL were obtained from Thermo Fisher, (Waltham, MA, USA). .. White, flat-bottom, high-binding 96-well plates (Greiner Bio-One, Kremsmünster, Austria, #655074) were coated overnight at 4 °C with 0.5 μg/mL recombinant human CD40 (R&D Systems, Minneapolis, MN, USA, #1493-CDB) in PBS. .. After washing with PBS/0.05% Tween 20 (PBST), the plates were blocked for 1 hour at room temperature using PBST with 2% BSA (Merck, Darmstadt, Germany, #1.12018.0100), followed by another wash. Antibodies were serially diluted in PBST/0.5% BSA (ranging from 14 to 0 μg/mL) and added to the plates, allowing them to bind for 1 hour at room temperature.

    Binding Assay:

    Article Title: Long-term immune responses to SARS-CoV-2 Omicron BA.4/5 mRNA booster in people living with HIV.
    Article Snippet: Total RBD-binding antibodies reactive against WT and BA.4/5 virus were measured in EDTA plasma samples by a homemade ELISA16,20,32. .. Briefly, high-binding 96-well plates (Greiner Bio-One, Cat# 655061) were coated with 3 μg/mL of recombinant wild-type SARS-CoV-2 receptor binding domain (RBD) [ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified), Cat# SPD-C52H3] or recombinant Omicron BA.4/5 SARS-CoV-2 RBD [ACROBiosystems SARS-CoV-2 Spike RBD Protein, His Tag (BA.4&BA.5/Omicron) (MALS verified), Cat# SPDC522r] diluted in 50mM of carbonate-bicarbonate buffer pH 9.6 (SigmaAldrich, Cat# C3041-50cap) and incubated overnight at+4 °C. .. Plates were then washed with washing buffer [PBS+ 0.05% TWEEN®20 (Sigma, Cat# P9416-100ML)] and blocked for 1.5 hours with blocking buffer [PBS+ 2% bovine serum albumin (BSA) (Sigma-Aldrich, Cat# A7030-100G)] at +37 °C.

    Multi-Angle Light Scattering:

    Article Title: Long-term immune responses to SARS-CoV-2 Omicron BA.4/5 mRNA booster in people living with HIV.
    Article Snippet: Total RBD-binding antibodies reactive against WT and BA.4/5 virus were measured in EDTA plasma samples by a homemade ELISA16,20,32. .. Briefly, high-binding 96-well plates (Greiner Bio-One, Cat# 655061) were coated with 3 μg/mL of recombinant wild-type SARS-CoV-2 receptor binding domain (RBD) [ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified), Cat# SPD-C52H3] or recombinant Omicron BA.4/5 SARS-CoV-2 RBD [ACROBiosystems SARS-CoV-2 Spike RBD Protein, His Tag (BA.4&BA.5/Omicron) (MALS verified), Cat# SPDC522r] diluted in 50mM of carbonate-bicarbonate buffer pH 9.6 (SigmaAldrich, Cat# C3041-50cap) and incubated overnight at+4 °C. .. Plates were then washed with washing buffer [PBS+ 0.05% TWEEN®20 (Sigma, Cat# P9416-100ML)] and blocked for 1.5 hours with blocking buffer [PBS+ 2% bovine serum albumin (BSA) (Sigma-Aldrich, Cat# A7030-100G)] at +37 °C.

    Incubation:

    Article Title: Long-term immune responses to SARS-CoV-2 Omicron BA.4/5 mRNA booster in people living with HIV.
    Article Snippet: Total RBD-binding antibodies reactive against WT and BA.4/5 virus were measured in EDTA plasma samples by a homemade ELISA16,20,32. .. Briefly, high-binding 96-well plates (Greiner Bio-One, Cat# 655061) were coated with 3 μg/mL of recombinant wild-type SARS-CoV-2 receptor binding domain (RBD) [ACROBiosystems SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified), Cat# SPD-C52H3] or recombinant Omicron BA.4/5 SARS-CoV-2 RBD [ACROBiosystems SARS-CoV-2 Spike RBD Protein, His Tag (BA.4&BA.5/Omicron) (MALS verified), Cat# SPDC522r] diluted in 50mM of carbonate-bicarbonate buffer pH 9.6 (SigmaAldrich, Cat# C3041-50cap) and incubated overnight at+4 °C. .. Plates were then washed with washing buffer [PBS+ 0.05% TWEEN®20 (Sigma, Cat# P9416-100ML)] and blocked for 1.5 hours with blocking buffer [PBS+ 2% bovine serum albumin (BSA) (Sigma-Aldrich, Cat# A7030-100G)] at +37 °C.

    Article Title: Temporal dynamics of SARS-CoV-2 antibodies and IgG subclasses following multiple doses and diverse COVID-19 vaccine combinations
    Article Snippet: .. High-binding 96-well plates (Greiner, Kremsmünster, Austria; #655061) were coated with SARS-CoV-2 Spike protein (Thermo Fisher, Waltham, USA; #RP-87700) diluted to 2 μg/ml in coating buffer (Thermo Fisher, #00-0044-59) at 50 μL per well and incubated overnight at 4°C. ..

    Concentration Assay:

    Article Title: Interfacial Interactions between Neural Tracing Lectin-Gold Nanoparticle Conjugate and Cell Membrane Glycoproteins.
    Article Snippet: .. Extracted glycoproteins were immobilized on high-binding 96-well plates (Greiner 655081) (50 μL per well) at a concentration of 4 μg/mL for 1 h at 37 °C on a shaker (Benchmark Multi-Therm). ..



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